Optimization of Lipase Activity from Pseudomonas Fluorescens Using Response Surface Methodology

Abstract

To ensure optimal lipase activity from Pseudomonas fluorescens, selection of various parameters for an optimized fermentation process is vital. The current study is aimed at the optimization of process parameters of the fermentation based on three basic process parameters during fermentation including pH, fermentation temperature and incubation hours so as to improve lipase activity using response surface methodology (RSM) coupled with central composite design method (CCD). As per the experimental data, correlation between the three investigated parameters with respect to lipase activity of Pseudomonas fluorescens was obtained. The results of analysis of variance (ANOVA) proved that the correlation proposed by quadratic regression was adequate for predicting lipase activity of Pseudomonas fluorescens. It was concluded that the lipase activity was significantly influenced by fermentation parameters. The fermentation temperature of 30 ⁰C, pH value of 8.5 and incubation of 30 hours were found to be optimal for optimal enzyme activity.